DNA sequence divergence and functional conservation at the STB locus of yeast 2 microns circle variants.

Rank microns circle variants . conservation at the STB locus of yeast 2 DNA

A cis-acting locus needed for stable maintenance of the yeast plasmid pSB3.

The Zygosaccharomyces rouxii plasmid pSB3 is one of the yeast plasmids resembling 2-microns DNA. The presence of a cis-acting locus needed for stable maintenance of pSB3 has been expected by analogy with the functions encoded by other yeast plasmids such as 2-microns DNA and pSR1. We found such a locus and designated it STB-SB3. The STB-SB3 locus was delimited within 371 bp in a unique region o...

DNA plasmid transmission in yeast is associated with specific sub-nuclear localisation during cell division.

Circular plasmids in yeast carrying only an origin of DNA replication (ARS) exhibit maternal inheritance bias (MIB) and are poorly transmitted from mother to daughter cell during division. A variety of different sequences that overcome MIB have been described, including centromeric sequences (CEN), telomere-associated repeats, silencer sequences and a specific system encoded by the endogenous 2...

Phylogenetic analysis of HSP70 gene of Aspergillus fumigatus reveals conservation intra-species and divergence inter-species

Aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecologicalniches and the most prevalent aspergilli responsible for human and animal invasiveaspergillosis. The first step to develop novel and efficient therapies is the identificationand understanding of the key tolerance and virulence factors of pathogens. The mainfocus of the present study is to perform the similarit...

The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-μm circle partitioning locus and promotes equal plasmid segregation

The centromere protein A homologue Cse4p is required for kinetochore assembly and faithful chromosome segregation in Saccharomyces cerevisiae. It has been regarded as the exquisite hallmark of centromeric chromatin. We demonstrate that Cse4 resides at the partitioning locus STB of the 2-microm plasmid. Cse4p-STB association is absolutely dependent on the plasmid partitioning proteins Rep1p and ...